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Quick-RNA™ MidiPrep
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10 minute method for isolating RNA (up to 125 µg) from a wide range of cell types and tissue samples. |
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Fast-Spin column technology allows ultra clean RNA to be eluted into minimal volumes (≥100 µl). |
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Omits the use of organic denaturants and proteases. |
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Formats: Spin column. |
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Sample Sources: Cells from culture or tissue samples, buffy coat, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of RNA from whole blood see the ZR Whole-Blood RNA MiniPrep (R1020)) |
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Sample Size: ~103 to 107 cells. |
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RNA Recovery: Up to 125 µg RNA can be eluted into ≥100 µl RNase-free water allowing for a highly concentrated sample. |
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RNA Purity: High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
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RNA Storage: -70ºC |
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Equipment Needed: Vacuum manifold, microcentrifuge. |
The Quick-RNA™ MidiPrep is an innovative product designed for the easy, reliable, and rapid isolation of up to ~125 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Fast-Spin column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ MidiPrep allows for the efficient recovery of total RNA from 103 to 107 cells or tissue (up to ~100 mg). The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
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PCR amplification of β-actin transcript (353 bp fragment shown) following RNA isolation from human epithelial cells (HCT 116) with the Quick-RNA™ MidiPrep kit: RT-PCR (lane 1, 2), PCR negative control (RNA template; lane 3, 4).
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