ZR BAC DNA Miniprep Kit
 
• For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion, etc. No messy precipitations!
• Innovative colored buffers* for rapid identification of complete bacterial cell lysis and neutralization steps.
• Unique column design: zero buffer retention and low-volume (≥10 μl) elution.
 
• Format: Spin Column
• Plasmid DNA Yield: Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. coli culture processed,and culture growth conditions. The typical yield is 1 μg per 3 ml of culture.
• Plasmid DNA Size: Up to 200 kb.
• Recovery Volume: ≥10 µl
• DNA Purity: High-purity and endotoxin-free (<50 EU/μg) plasmid DNA in low salt buffer or water; typical A260/280 ≥ 1.8. Eluted DNA is suitable for sequencing, endonuclease digestion, PCR, and other reactions requiring highly purified DNA.
• Procedure: Can be conducted at room temperature, between 15-30°C.
 

The ZR BAC DNA Miniprep Kit is for efficient isolation of BAC plasmid DNA or other large plasmids (e.g., PAC) from E. coli cell lysates using a procedure that is simple, rapid, user-friendly, and reliable compared to kits offered from other suppliers. It features a modified alkaline lysis protocol together with unique spin column matrix technology to yield high quality plasmid DNA in minutes. The ZR BAC DNA Miniprep Kit features color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and buffer neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is well suited for sequencing, PCR, endonuclease digestion, etc.

 

Figure 1. Digestion of BAC DNA with HindIII and NotI endonucleases. A BAC clone (~160 kb) from a RPCI-11 human BAC library (CHORI) was purified from DH10B electrocompetent cells (Invitrogen) using the ZR BAC DNA Miniprep Kit. Digestion with NotI removed the insert (~148 kb) from the pBACe3.6 cloning vector1 (11.6 kb) indicated by the (◄) in the gel image above. M is a 1 kb DNA ladder (Zymo Research)
 
Product Catalog No. Size Price Store
ZR BAC DNA Miniprep Kit D4048 25 Preps. $
  D4049 100 Preps. $
 
Components Available