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ZR-Duet™ DNA/RNA MiniPrep
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Quick (15 minute) isolation and separation of genomic DNA and total RNA (up to ~25 μg) from a wide range of sources using Fast-Spin column technology. |
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DNA/RNA eluted into volumes ≥25 μl is suitable for use in PCR, RT-PCR and other procedures. |
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Omits the use of organic denaturants, β-mercaptoethanol, and proteases. |
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Format: Spin Column |
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Sample Sources: Cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of DNA and RNA from whole blood see the Quick-gDNA™ MiniPrep (D3024) and the ZR Whole-Blood RNA MiniPrep (R1020)) |
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Sample Size: 102 to 5x106 cells in suspension or as tissue. |
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DNA/RNA Recovery: up to 25 μg DNA/RNA can be eluted into ≥25 μl. |
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Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. Total RNA ≥17 nucleotides can be recovered. |
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Purity: High quality genomic DNA and total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. |
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RNA Storage: -70°C |
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Equipment Needed: Microcentrifuge |
The ZR-Duet™ DNA/RNA MiniPrep provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes.
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Figure 1. Genomic DNA (lane 1, 2) and total RNA (lane 3, 4) isolated from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep.
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Figure 2. PCR amplification of β-actin transcript (353bp fragment shown) following DNA and RNA isolation from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep: PCR positive control (DNA template; lane 1, 2, 3), PCR negative control (RNA template; lane 4, 5, 6), RT-PCR (lane 7, 8, 9).
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| Product |
Catalog No. |
Size |
Price |
Store |
ZR-Duet™ DNA/RNA MiniPrep
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D7001 |
50 Preps. |
$
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| Components Available
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