About ZR BAC DNA Miniprep Kit
The ZR BAC DNA Miniprep Kit is for the efficient isolation of BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 15 min |
| DNA Purity | High-purity and endotoxin-free (<50 EU/μg) plasmid DNA in low salt buffer or water; typical A260/280 ≥ 1.8. Eluted DNA is suitable for sequencing, endonuclease digestion, PCR, and other reactions requiring highly purified DNA. |
| Plasmid DNA Size | 50 bp to 200 kb. |
| Plasmid DNA Yield | Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed,and culture growth conditions. The typical yield is 1 μg per 3 ml of culture. |
| Procedure | Can be conducted at room temperature, between 15-30°C. |
| Recovery Volume | ≥ 10 µl |
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HindIII and NotI digestion of BAC DNA. A BAC (~160 kb) from a RPCI-11 human BAC library (CHORI) was purified from DH10B cells (Invitrogen) using the ZR BAC DNA Miniprep Kit. Digestion with NotI removed the ~148 kb insert from the 11.6 kb pBACe3.6 cloning vector 1 (?). M: 1 kb DNA ladder (Zymo Research).
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