About Zymoclean™ Gel DNA Recovery Kit
The Zymoclean™ Gel DNA Recovery Kit provides for the rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. The product features Fast-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery kit are perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge |
| DNA Size Limits | 50 bp to 23 kb |
| Sample Sources | DNA excised from TAE/TBE-buffered agarose gels. |
| DNA Purity | High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.
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DNA sequencing chromoatogram of a PCR product recovered using the Zymoclean™ Gel DNA Recovery Kit. DNA was recovered from a 2% (w/v) agarose gel and used directly for sequencing.
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