About DNA Clean & Concentrator™-100
The DNA Clean & Concentrator™-100 (DCC™-100) is designed for the rapid desalting and purification of up to 100 µg of high quality DNA from PCR, large format restriction endonuclease digestions, or cell-free lysates. Eluted DNA is suitable for nucleotide sequencing, array analysis, PCR, nucleotide blotting, restriction endonuclease digestion procedures, as well as many other downstream applications requiring high quality DNA. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a syringe, centrifuge or vacuum source together with a microcentrifuge.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, ≤100 µg total DNA can be eluted with ≥100 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment | Microcentrifuge and centrifuge, vacuum source, or syringe. |
| DNA Size Limits | 50 bp to 23 kb |
| Sample Sources | DNA from PCR, endonuclease digestions, and cell-free DNA preps., etc. |
| DNA Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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I feel the DNA Clean and Concentrator Kit is a perfect choice for efficient DNA clean up which can translate to good, reproducible results in several downstream applications.-Akshat Mathur
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