About DNA Clean & Concentrator™-25
The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product features Fast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment | Microcentrifuge |
| DNA Size Limits | 50 bp to 23 kb |
| Sample Sources | DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| DNA Purity | High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.
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Protocol (226.7 KB)
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MSDS (104.6 KB)
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Data Sheet (1014 KB)
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I feel the DNA Clean and Concentrator Kit is a perfect choice for efficient DNA clean up which can translate to good, reproducible results in several downstream applications.-Akshat Mathur
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