About DNA Clean & Concentrator™-500
The DNA Clean & Concentrator™-500 (DCC™-500) is our highest capacity DNA Clean & Concentrator™ product. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.
Specifications
| Format | Spin Column |
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| DNA Recovery | Typically, ≤ 500 µg total DNA can be eluted with ≥ 1 ml water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70% |
| Equipment | Microcentrifuge and centrifuge, vacuum source, or syringe |
| DNA Size Limits | From 50 bp to 23 kb |
| Sample Sources | DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates |
| DNA Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions |
| Product Detergent Tolerance | ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS |
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Ultra-pure DNA from the DNA Clean & Concentrator™ can be easily ligated for efficient cloning. Lanes: A: 3.5 kb blunt-ended vector; B: 7.4 kb blunt-ended DNA insert; C: Blunt-end ligation of A+B mixture. |
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I feel the DNA Clean and Concentrator Kit is a perfect choice for efficient DNA clean up which can translate to good, reproducible results in several downstream applications.-Akshat Mathur
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