About ZR-96 DNA Clean-up Kit™
The ZR-96 DNA Clean-up Kit™ provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A™ Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.
Specifications
| Format | 96-well Plate |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA (per well) can be eluted into as little as 30 - 40 µl water per sample. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment | Centrifuge (with microplate carriers) |
| DNA Size Limits | 50-10kb |
| Sample Sources | DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| DNA Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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High-throughput DNA processing. Crude preparations of a 3 kb plasmid DNA from bacterial lysates were processed using the ZR-96 DNA Clean-up Kit™. Following elution from the plate, 48 samples were then separated in a 0.8% (w/v) agarose gel.
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I tried this kit for the first time and I was truly amazed at how fast and easy it is to use.-Vincent Magrini, Ph.D
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