About ZR-96 Fungal/Bacterial DNA Kit™
The ZR-96 Fungal/Bacterial DNA Kit™ is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin plate technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Specifications
| Format | 96-well Plate |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time | 40 min |
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer. |
| DNA Size Limits | Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered. |
| Sample Sources | 10-20 mg (wet weight) fungi or bacteria. This equates to approximately 2x108 bacterial cells, 2x107 yeast cells and 2x106 mammalian cells. Spores, pollen, nematodes, other microorganisms, and up to 40 mg soft tissue (e.g., mammalian) can also be sampled. |
| DNA Purity | High quality DNA is eluted with DNA Elution Buffer making it perfect for PCR (A260/A280 nm >1.8. |
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Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. Coli using the ZR Fungal/Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).
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