About ZR Fungal/Bacterial DNA MicroPrep™
The ZR Fungal/Bacterial DNA MicroPrep™ is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA is eluted into ≥ 10 µl DNA Elution Buffer per sample. |
| Processing Time | 10 min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | 10-20 mg (wet weight) fungi or bacteria or up to 40 mg tissue. This equates to approximately 2x108 bacterial cells, 2x107 yeast cells and 2x106 mammalian cells. Spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| DNA Purity | High quality DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
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Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. Coli using the ZR Fungal/Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).
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