About ZR Serum DNA Kit™
The ZR Serum DNA Kit™ is based on a state of the art, single buffer procedure for rapid DNA isolation from large volume serum and plasma samples. The product recovers genomic, mitochondria, and viral DNAs having typical sizes from 25 kb to 50 kb without RNA contamination. The uniquely formulated Genomic Lysis Buffer efficiently lyses cells, virus, and/or cellular particles. DNA/ZymoBead™ complexes are separated by centrifugation, and then washed to remove contaminants. Eluted, purified DNA is ideal for PCR and other sensitive analytical procedures.
|DNA Recovery||Scalable to compensate for smaller/larger sample sizes.|
|Equipment||Microcentrifuge or centrifuge (depending on sample volume).|
|DNA Size Limits||>1 kb|
|Sample Sources||Tissue, buccal cells, cells from culture, biological liquids, and blood from humans, mice, rabbits, etc.|
|DNA Purity||Purified, genomic DNA is recovered in water which is especially good for subsequent experiments. Mitochondrial and viral DNA will also be recovered.|
Ensure that the ZymoBeads™ are resuspended by vortexing. In a conical 50 ml tube add 4 volumes of Genomic Lysis Buffer to each volume of sample (4:1) and then add 10 µl of ZymoBeads™.
Example #1: 800 µl of plasma, add 3.2 ml of Genomic Lysis Buffer and 10 µl of ZymoBeads™.
Example #2: 5.0 ml of serum, add 20 ml of Genomic Lysis Buffer and 10 µl of ZymoBeads™.
Mix by placing sample on a rotator/rocker for 2 hours at room temperature or overnight at 0-4ºC.
|Step 3||Centrifuge tube for 1-2 minutes. Discard the supernatant.|
Add 500 µl of DNA Wash Buffer to the ZymoBeads™. Resuspend the pellet and then transfer mixture to a 1.5 ml microcentrifuge tube.
|Step 5||Centrifuge for 1 minute in a microcentrifuge. Discard the supernatant.|
Add another 500 µl of DNA Wash Buffer to the ZymoBeads™. Resuspend the pellet and centrifuge for 1 minute. Discard the supernatant.
|Step 7||Recentrifuge briefly and remove any residual wash buffer. Air-dry the pellet for 15 minutes.|
Add 10-35 µl DNA Elution Buffer or water to the ZymoBeads™ and resuspend by repeated pipetting.
|Step 9||Centrifuge at 10,000 x g for 1 minute.|
|Step 10||Collect the supernatant which contains the purified DNA. The DNA can be used immediately or stored at -20ºC for later use.|