About Quick-gDNA™ MiniPrep
The Quick-gDNA™ MiniPrep is for the convenient, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and buccal cells, cells from culture, and many biological liquid samples can be processed with these kits. The product features Fast-Spin column technology for high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 25 µg total genomic DNA is eluted into ≥ 50 µl DNA Elution Buffer or water. Human whole blood will yield 33 ng - 66 ng DNA per µl blood sampled. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex. |
| DNA Size Limits | Capable of recovering genomic DNA >40 kb. In most instances, mitochondrial DNA is also recovered. |
| Sample Sources | Whole blood, plasma, or serum from humans, mice, rats, etc. Also, tissue, cells from culture, buccal cells, as well as a variety of liquid samples are effectively processed using this kit. |
| DNA Purity | High-quality DNA is eluted with DNA Elution Buffer or water that is especially well suited for PCR and other downstream applications. Typical absorption indices are A(260/280nm) >1.8. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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DNA isolated from porcine whole blood using the Quick-gDNA™ MiniPrep. Equivalent amounts (100 µl) of blood were processed without Proteinase K using the Quick-gDNA™ MiniPrep in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research).
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