Xpedition™ Soil/Fecal DNA MiniPrep

Degradation and contamination of biological samples have been obstacles to scientific study, and may be particularly problematic in highly sensitive molecular-analysis techniques (e.g., PCR of low copy DNA). Use of cryogenic freezing methods for environmental/forensic sample preservation may often be too impractical to be employed. The Xpedition™ Soil/Fecal DNA MiniPrep, when used with a portable bead beating device [i.e., Xpedition™ Sample Processor (XSP), pg. 6], allows the researcher/investigator to "Take the Lab to the Field" and to the site of sample collection. The Xpedition™ Soil/Fecal DNA MiniPrep can be used for the isolation of inhibitor-free DNA from tough-to-lyse bacteria, fungi, protozoa, algae, etc., that inhabit a range of samples including feces as well as clay, sandy, salty, silty, peaty, chalky, and loamy soils. Samples from remote locations can be processed at the site of collection using the provided ZR BashingBead™ Lysis Tubes and a XSP (available separately, Cat. No. S6020, pg. 6). Sample processing occurs in a unique lysis/stabilization solution that effectively preserves DNA in soil/fecal lysates for subsequent storage and transport. Later, when convenient with the researcher, the kit's Fast-Spin column technologies are used for the isolation of humic acid/polyphenol-free DNA from the lysates. This product can also be used for isolation of DNA from cultured bacteria, fungi, and yeast. Eluted DNA is ideal for PCR for various genotypic analysis including metagenomics, forensics, barcoding, phylogenetics, etc. An overview of the procedure is shown below.