About ZR-96 Fecal DNA Kit™
The ZR-96 Fecal DNA Kit™ is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin plate technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
Specifications
| Format | 96-well Plate |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time | 50 min |
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer. |
| DNA Size Limits | Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 kb "“ 35 kb. If present, parasitic and viral DNA will also be recovered. |
| Sample Sources | Bacterial, protist, as well as host DNA can be effectively isolated from a ≤80 mg sample of mammalian feces. |
| DNA Purity | High quality, inhibitor-free DNA is eluted with DNA Elution Buffer suitable for the amplification of bacterial, protist, and/or mammalian templates. Absorbance 260 nm/280 nm >1.8. |
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Comparison of DNA yields from rat feces using the ZR Fecal DNA MiniPrep™ and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.
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