About ZR Fecal DNA MicroPrep™
The ZR Fecal DNA MicroPrep™ is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Up to 5 μg total DNA is eluted into ≥ 10 μl DNA Elution Buffer per sample. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional) |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | Bacterial, protist, as well as host DNA can be effectively isolated from a ≤150 mg sample of mammalian feces. |
| DNA Purity | High quality, inhibitor-free DNA is eluted with DNA Elution Buffer suitable for the amplification of bacterial, protist, and/or mammalian templates. A260/A280 >1.8 |
 |
Comparison of DNA yields from rat feces using the ZR Fecal DNA MiniPrep™ and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.
|
-
The ZR Fecal DNA kit was identified as affording the greatest analytical sensitivity and reproducibility and was able to detect 10 T. foetus organisms per 100 mg feces in 100% of PCR reactions. Further, the identified extraction method could be completed-Stauffer, SH et al, Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 5, 639-641
- Submit Your Feedback
Your shopping cart is empty.
