About ZR FFPE DNA MiniPrep™
The ZR FFPE DNA MiniPrep™ provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Fast-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as given in the protocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc. Shown below is a schematic and performance overview of the procedure.
Specifications
| DNA Recovery | High quality total DNA (A260/A280 >1.8) can be eluted into small volumes (i.e., ≥25 µl) allowing for highly concentrated samples. The maximum DNA binding capacity of the provided spin column is ~25 µg. |
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| Processing Time | As little as 4 hours when processing large amounts of tissue. For maximum yields of the highest quality DNA, it is recommended to process samples overnight. |
| Sample Size | Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm2. It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens. |
| Equipment/Reagents | Microcentrifuge, thermomixer or heat block/bath capable of 55°C and 90°C, xylene, ethanol, isopropanol, beta-mercaptoethanol (optional). |
