DNA PURIFICATION OVERVIEW

The fidelity of the method used for the isolation/purification of DNA from biological samples or from reaction mixtures is of critical importance when considering the success of subsequent downstream molecular applications. Many molecular-based applications including PCR, Southern blotting, automated DNA sequencing, microarray, etc., require that the DNA purification procedure result in high yields of high quality DNA.

With this considered, the scientists at Zymo Research have developed a range of DNA isolation kits designed for the simple and rapid recovery of high yield, high quality DNA from a diversity of sample sources.

DNA Fragment Clean-up

The clean-up and concentration of DNA fragments is vital for the success of downstream processing such as sequencing, cloning, ligation, microarray and endonuclease digestion procedures. The process involves the rapid desalting, purifying and concentrating of DNA from in vitro reaction mixtures and cell-free lysates. Zymo Research Corporation has perfected this process to produce a fast, simple and efficient way of “cleaning” DNA fragments. This kit produces a high quality yield, perfect for your downstream molecular applications.

Plasmid DNA Purification

The Zyppy™ plasmid DNA purification kits allow the user to separate plasmid DNA efficiently from chromosomal DNA and cellular RNA in bacterial host cell lysates using procudures that are fast, user-friendly, and reliable when compared to those offered by other suppliers.

Genomic DNA Isolation

Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh and paraffin-embedded tissue sections, cultured cells, saliva, buccal cells, whole blood, plasma, serum, urine, bacteria, fungi, yeast, algae, viruses, and mitochondria.

DNA Molecular Weight Markers

The ZR DNA Markers™ are created as defined size fragments that encompass a range of sizes from 50 bp up to 10 kb. This makes DNA size approximation easy for both PCR products as well as plasmid DNAs.