DNA PURIFICATION OVERVIEW
The fidelity of the method used for the isolation/purification of DNA from biological samples or from reaction mixtures is of critical importance when considering the success of subsequent downstream molecular applications. Many molecular-based applications including PCR, Southern blotting, automated DNA sequencing, microarray, etc., require that the DNA purification procedure result in high yields of high quality DNA.
With this considered, the scientists at Zymo Research have developed a range of DNA isolation kits designed for the simple and rapid recovery of high yield, high quality DNA from a diversity of sample sources.
DNA Purification Categories
The clean-up and concentration of DNA fragments is vital for the success of downstream processing such as sequencing, cloning, ligation, microarray and endonuclease digestion procedures.
The Zyppy™ plasmid DNA purification kits allow the user to separate plasmid DNA efficiently from chromosomal DNA and cellular RNA in bacterial host cell lysates using procedures that are fast and reliable.
Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types.
The ZR DNA Markers™ are created as defined size fragments that encompass a range of sizes from 50 bp up to 10 kb. This makes DNA size approximation easy for both PCR products as well as plasmid DNAs.
Featured DNA Purification Products
The DNA Clean & Concentrator™-5 product provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
The OneStep™ PCR Inhibitor Removal Kit contains all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations.
The ZR FFPE DNA MiniPrep™ provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections.