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OneStep™ PCR Inhibitor Removal Kit

Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.

Product Size Catalog # Price Qty
OneStep™ PCR Inhibitor Removal Kit 50 Preps. D6030

About OneStep™ PCR Inhibitor Removal Kit

The OneStep™ PCR Inhibitor Removal Kit contains all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column.

Format Spin Column
DNA Recovery 50 - 90%
Processing Time 5 min
Elution Volume 50 - 200 µl
RNA Recovery 50 - 90%

DNA is efficiently amplified by PCR following humic acid removal with the OneStep™PCR Inhibitor Removal Kit. The figure shows amplification of a 200 bp product from DNA containing humic acid that was treated with the kit. Alternatively, PCR amplification was completely inhibited in the case of the non-treated sample. In each case, equal amounts of DNA were used for each PCR and equivalent amounts of the reaction were then analyzed in a 2.0% (w/v) agarose/TAE/EtBr gel. The ladder is a 100 bp DNA marker (Zymo Research). Hot start PCR was performed using ZymoTaq™ PreMix (Zymo Research)

PCR amplification of an eukaryotic transcript (post-RT): Total RNA isolated from sludge with or without inclusion of the Zymo-Spin™ IV-HRC Spin Filter. M is a 1 kb DNA Marker (Zymo Research).

Step 1

Snap off the base

Step 2

Remove the cap

Step 3

Insert into a Collection Tube

Step 4

Spin in a microcentrifuge at exactly 8,000 x g for 3 minutes. 

Note: If the HRC matrix is dry, add 400-600 µl water prior to prepping the column.

For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

The OneStep™ PCR Inhibitor Removal Kit was used to purify total RNA extracted from B. neritina colonies for sequence and phylogenetic studies. Analysis of the transcriptome suggests that symbiont-produced bryostatins are involved in reproduction of host B. neritina via PKC activation.
Melanin was removed from melanocyte DNA and RNA using the OneStep™ PCR Inhibitor Removal Kit, and pigment phenotype was determined by single nucleotide polymorphisms by sequencing on Illumina platforms. Results showed significant heterogeneity in gene expression in response to fibroblast-conditioned medium.

“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”
Tara N. (United States Agricultural Research Service)
“The ease. Holy cow, this thing was so freaking simple compared to similar products”
Christian H. (University of Georgia)
Click here to submit your review of the OneStep™ PCR Inhibitor Removal Kit.

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