DNA Clean & Concentrator™-25

Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Product Size Catalog # Price Qty
DNA Clean & Concentrator™- 25 - Uncapped Columns 50 Preps D4005
$75.00
DNA Clean & Concentrator™- 25 - Uncapped Columns 200 Preps D4006
$272.00
DNA Clean & Concentrator™- 25 - Capped Columns 50 Preps D4033
$75.00
DNA Clean & Concentrator™- 25 - Capped Columns 200 Preps D4034
$272.00

About DNA Clean & Concentrator™-25

The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product features Fast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.


Format Spin Column
DNA Recovery Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
Equipment Microcentrifuge
DNA Size Limits 50 bp to 23 kb
Sample Sources DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc.
DNA Purity High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.

Step 1

In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below).  Mix briefly by vortexing.

Application

DNA Binding Buffer  :  Sample

Example

Plasmid, genomic DNA (>2 kb)

2  :  1

200 µl  :  100 µl

PCR product, DNA fragment

5  :  1

500 µl  :  100 µl

ssDNA1 (e.g. cDNA, M13 phage)

7  :  1

700 µl  :  100 µl

For efficient recovery of genomic or large DNA (< 20 kb to < 200 kb), use the Genomic DNA Clean &  Concentrator™ (Cat. Nos. D4010, D4011).

Step 2

Transfer mixture to a provided Zymo-Spin™ Column2 in a Collection Tube.

Step 3

Centrifuge for 30 seconds.  Discard the flow-through.

Step 4

Add 200 µl DNA Wash Buffer to the column.  Centrifuge at for 30 seconds.  Repeat the wash step.

Step 5

Add ≥ 25 µl DNA Elution Buffer3 or water4 directly to the column matrix and incubate at room temperature for one minute.  Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge at for 30 seconds to elute the DNA.

Ultra-pure DNA is now ready for use.

For specific notes and additional information, please see the product protocol PDF.
 

Product FAQs

  • Can you show it can clean up 50 bp fragments?

  • Can I use lower volumes of DBB for recovery of ss cDNA?

    Two volume of Binding Buffer works fine in most situation.  However, due to different buffer recipes and pH, especially after NaOH degradation of RNA in cDNA synthesis, we recommend to use up to 7 volumes of binding buffer for consistent yield and performance. There is no change in buffer and other components for this kit.

  • I accidentally added 2 vol. of EtOH instead of DBB to my samples (100 ul sample + 200 ul EtOH). What should I do?

    Add 2 vol additionally of DBB to the mixture.

  • My samples are floating out of my gels, why and how can I prevent it?

    Incomplete centrifugation of the columns after adding the wash buffer will cause this problem. Increase the centrifuge time (up to 1 minute or more) at maximum speed to ensure complete removal of the ethanol from the column.

  • I'm getting low recovery

    Improperly Prepared/Stored DNA Wash Buffer  

    Make sure ethanol has been added to the DNA Wash Buffer concentrate.  Cap the bottle tightly to prevent evaporation over time.

    Addition of DNA Elution Buffer

    Add elution buffer directly to the column matrix and not to the walls of the column.  Elution buffer requires contact with the matrix for at least 1 minute for large DNA ≥ 10 kb.

    Incomplete Elution

    DNA elution is dependent on pH, temperature, and time.  For large genomic DNA (≥ 50 kb), apply heated elution buffer (60-70 °C) and incubate for several minutes prior to elution.

    Sequential elutions may be performed for quantitatively higher recovery but lower final DNA concentration.  This is recommended for DNA ≥ 10 kb.

  • My A260/A230 Ratios are low

    Column Tip Contaminated

    When removing the column from the collection tube, be careful that the tip of the column does not come into contact with the flowthrough.  Trace amounts of salt from the flowthrough can contaminate a sample resulting in low A260/A230 ratios.  Ethanol contamination from the flowthrough can also interfere with DNA elution.  Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover.

  • Following Clean-up with the DCC™, Multiple Bands Appear in an Agarose Gel

    Acidification of DNA Loading Dye

    Most loading dyes do not contain EDTA and will acidify (pH ≤ 4) over time due to some microbial growth. This low pH is enough to cause DNA degradation.  Therefore, if water is used to elute the DNA, 6X Loading Dye containing 1 mM EDTA is recommended.

  • I am experiencing poor recoveries, do you have any tips?

    1)  Ensure that your entire sample flows through the column after each centrifugation.

    2)  Remember to empty the collection tube when advised.

    3)  Be careful that the tip of the column does not come into contact with the flowthrough when removing the column from the collection tube.

    4)  Extend the final wash spin by 1-2 minutes to ensure complete wash buffer removal.

    5)  Add your elution buffer directly to the column matrix, not to the walls of the column.

Submit your Citation
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Featured Citations

The DNA Clean & Concentrator™-25 was used to purify cDNA library for Illumina small RNA deep sequencing in the analysis of miRNA transfected cells. Results showed varying concentrations of miRNA mimics caused non-specific alterations in gene expression and that guide strands were frequently mutated.
PCR products were purified using the DNA Clean & Concentrator™-25, followed by Sanger multilocus sequence analysis and confirmed by repetitive PCR; this revealed the high diversity of C. michiganensis subspecies, an agent of bacterial canker of tomato.
The DNA Clean & Concentrator™-25 was used to purify genomic DNA from different phytoplankton species extracted using a CTAB/chloroform method. Researchers found that bead-beating improved DNA yield by as much as 2 fold and that DNA integrity was preserved by subsequent qPCR analysis and cloning of the ITS region.
In a study of common viruses infecting cucurbits, the DNA Clean & Concentrator-25™ was used to clean PCR products amplified from reverse transcribed viral RNA prior to cloning. Specific viruses were categorized into a phylogenetic relationship and can lead to better virus control by means of genetic engineering.
High-quality amplified cDNA was obtained using the DNA Clean & Concentrator™-25 (DCC™-25) following microarray analysis. Purification helped determine the off-targeting potential of potentially therapeutic RNAi constructs.

“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
Rosalind P. (University of Arkansas for Medical Sciences)
“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner. The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.”
Joseph R. (Miller School of Medicine, University of Miami)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
Takashi K. (Monash University)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
Kimberly M. (University of Pennsylvania)
“It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.”
Marissa V. (Harvard Medical School)
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
Shanshan L. (UT Southwestern)
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”
Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
Tyler C.
Click here to submit your review of the DNA Clean & Concentrator™-25 .

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