DNA Clean & Concentrator™-500

Simple, rapid recovery of ultra-pure DNA from large-scale sample sources.
Unique column construction allows sample washing to be performed using a centrifuge or vacuum source.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Product Size Catalog # Price Qty
DNA Clean & Concentrator™- 500 10 Preps D4031
$75.00
DNA Clean & Concentrator™- 500 20 Preps D4032
$138.00

About DNA Clean & Concentrator™-500

The DNA Clean & Concentrator™-500 (DCC™-500) is our highest capacity DNA Clean & Concentrator™ product. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.


Format Spin Column
DNA Recovery Typically, ≤ 500 µg total DNA can be eluted with ≥ 1 ml water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%
Equipment Microcentrifuge and centrifuge, vacuum source, or syringe
DNA Size Limits From 50 bp to 23 kb
Sample Sources DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates
DNA Purity Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions
Product Detergent Tolerance ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS

 
 

Ultra-pure DNA from the DNA Clean & Concentrator™ can be easily ligated for efficient cloning. Lanes: A: 3.5 kb blunt-ended vector; B: 7.4 kb blunt-ended DNA insert; C: Blunt-end ligation of A+B mixture.

 

Product FAQs

  • Can you show it can clean up 50 bp fragments?

  • Can I use lower volumes of DBB for recovery of ss cDNA?

    Two volume of Binding Buffer works fine in most situation.  However, due to different buffer recipes and pH, especially after NaOH degradation of RNA in cDNA synthesis, we recommend to use up to 7 volumes of binding buffer for consistent yield and performance. There is no change in buffer and other components for this kit.

  • I accidentally added 2 vol. of EtOH instead of DBB to my samples (100 ul sample + 200 ul EtOH). What should I do?

    Add 2 vol additionally of DBB to the mixture.

  • My samples are floating out of my gels, why and how can I prevent it?

    Incomplete centrifugation of the columns after adding the wash buffer will cause this problem. Increase the centrifuge time (up to 1 minute or more) at maximum speed to ensure complete removal of the ethanol from the column.

  • I'm getting low recovery

    Improperly Prepared/Stored DNA Wash Buffer  

    Make sure ethanol has been added to the DNA Wash Buffer concentrate.  Cap the bottle tightly to prevent evaporation over time.

    Addition of DNA Elution Buffer

    Add elution buffer directly to the column matrix and not to the walls of the column.  Elution buffer requires contact with the matrix for at least 1 minute for large DNA ≥ 10 kb.

    Incomplete Elution

    DNA elution is dependent on pH, temperature, and time.  For large genomic DNA (≥ 50 kb), apply heated elution buffer (60-70 °C) and incubate for several minutes prior to elution.

    Sequential elutions may be performed for quantitatively higher recovery but lower final DNA concentration.  This is recommended for DNA ≥ 10 kb.

  • My A260/A230 Ratios are low

    Column Tip Contaminated

    When removing the column from the collection tube, be careful that the tip of the column does not come into contact with the flowthrough.  Trace amounts of salt from the flowthrough can contaminate a sample resulting in low A260/A230 ratios.  Ethanol contamination from the flowthrough can also interfere with DNA elution.  Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover.

  • Following Clean-up with the DCC™, Multiple Bands Appear in an Agarose Gel

    Acidification of DNA Loading Dye

    Most loading dyes do not contain EDTA and will acidify (pH ≤ 4) over time due to some microbial growth. This low pH is enough to cause DNA degradation.  Therefore, if water is used to elute the DNA, 6X Loading Dye containing 1 mM EDTA is recommended.

  • I am experiencing poor recoveries, do you have any tips?

    1)  Ensure that your entire sample flows through the column after each centrifugation.

    2)  Remember to empty the collection tube when advised.

    3)  Be careful that the tip of the column does not come into contact with the flowthrough when removing the column from the collection tube.

    4)  Extend the final wash spin by 1-2 minutes to ensure complete wash buffer removal.

    5)  Add your elution buffer directly to the column matrix, not to the walls of the column.

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“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
Rosalind P. (University of Arkansas for Medical Sciences)
“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner. The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.”
Joseph R. (Miller School of Medicine, University of Miami)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
Takashi K. (Monash University)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
Kimberly M. (University of Pennsylvania)
“It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.”
Marissa V. (Harvard Medical School)
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
Shanshan L. (UT Southwestern)
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”
Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
Tyler C.
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