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Oligo Clean & Concentrator™

Quick (2 minute) recovery of ultra-pure DNA and RNA oligonucleotides.
Complete removal of dyes, salts, enzymes, nucleotides, and short oligos.
≥6 µl elution with zero retention Fast Spin columns.
Eluted DNA/RNA is well suited for use in hybridization, sequencing, PCR, ligation, etc.

Product Size Catalog # Price Qty
Oligo Clean & Concentrator™ 50 Preps. D4060
Oligo Clean & Concentrator™ 200 Preps. D4061

About Oligo Clean & Concentrator™

The Oligo Clean & Concentrator™ provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure.

There is no need for organic denaturants or chloroform. Instead, the kit features Fast Spin column technology and employs a single-buffer system that allows for efficient DNA/RNA adsorption to the matrix of Zymo-Spin™ IC Column. DNA/RNA is washed and concentrated into a small volume of water (≥6 µl). Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.

Sample Sources Enzymatic reaction mixtures containing DNA/RNA fragments (radioactive-, biotin-, DIG-labeled) and oligonucleotides ≥16 nt.
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Recovery Volume Binding capacity of the Zymo-Spin™ IC column is 10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery >90 %. The column can be eluted with ≥6 µl.
Compatibility Single-straded (ss) and double-stranded (ds) DNA and RNA.
Purity High-quality DNA/RNA (A260/A280 nm <1.8; A260/A230 nm <1.8) eluted with water is especially well suited for hybridization, sequencing, ligation, and PCR.
Size Limit For oligonucletides ≥16 nt, up to 23 kb.

The Oligo Clean & Concentrator™ facilitates >90% recovery of ssDNA oligonucleotides (A) and efficient short oligo and nucleotide removal (B).

Step 1

Add 100 µl Oligo Binding Buffer to 50 µl sample1.

Step 2

Add 400 µl ethanol2 (95-100%), mix briefly by pipetting and transfer the mixture to a provided Zymo-Spin™ Column3 in a Collection Tube.

Note:  If required, scale up volumes proportionally.

Step 3

Centrifuge at ≥10,000 x g for 30 seconds.  Discard the flow-through.

For radioactive samples:  Transfer the column into a new collection tube then discard the tube containing the radioactive flow-through appropriately.

Step 4

Add 750 µl DNA Wash Buffer to the column.  Centrifuge at ≥10,000 x g for 30 seconds and discard the flow-through.  Then centrifuge at maximum speed for 1 minute.

Step 5

Transfer the column to a microcentrifuge tube. Add 15 µl water4 directly to the column matrix and centrifuge at ≥10,000 x g for 30 seconds to elute the oligonucleotide. Ultra-pure oligonucleotide in water is now ready for use.

For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

The Oligo Clean & Concentrator™ was used to purify small DNA fragments after restriction enzyme digestion, as a part of a CRISPR library generation protocol. A large number of RNA guide libraries were constructed to label repetitive loci in Xenopus egg extracts for an efficient method of whole genome screening.

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