About ZR-96 ChIP DNA Clean & Concentrator™
The ZR-96 ChIP DNA Clean & Concentrator™ (ZR-96 ChIP DCC™) provides a hassle-free method for the rapid, high throughput purification and concentration of high-quality DNA from any step in a "standard" ChIP protocol. This includes samples that have: A) undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical/enzymatically-mediated DNA shearing or B) reverse cross-linked samples eluted from chromatin-antibody-bead complexes. Additionally, this product may also be used to purify DNA from PCR and other enzymatic reactions. DNA purified using the ZR-96 ChIP DCC™ is suitable for PCR, Next-Gen sequencing (ChIP-Seq), arrays, as well as other molecular applications.
|DNA Recovery||Typically, up to 5 µg total DNA (per well) can be eluted into as little as 10 µl of low salt DNA Elution Buffer or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%.|
|DNA Size Limits||From ~50 bp to 23 kb.|
|Sample Sources||Any step in a standard ChIP protocol including:
a) Samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical or enzymatically-mediated DNA shearing.
b) Reverse cross-linked samples eluted from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS.
Note: This kit can also be used for DNA purification from PCR, restriction digests, kinase, phosphatase and other enzymatic reactions.
|DNA Purity||High-quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS.|
Note: All centrifugation steps should be performed between 3,000 - 5,000 x g.