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ZR-96 DNA Clean & Concentrator™-5 (Deep well format)

Clean and concentrate up to 5 μg DNA (per well) with ≥ 10 μl elution volume in as little as 15 minutes with 0 μl wash residue carryover.
Deep-well filtration plate designs allow DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.

Product Size Catalog # Price Qty
ZR-96 DNA Clean & Concentrator™-5 - Deep-well 2x96 Preps. D4023
$220.00
ZR-96 DNA Clean & Concentrator™-5 - Deep-well 4x96 Preps D4024
$420.00

About ZR-96 DNA Clean & Concentrator™-5 (Deep well format)

The ZR-96 DNA Clean & Concentrator™-5 products provide purification of up to 5 µg DNA per well from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. The product facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.


Format 96-well Plate
DNA Recovery Typically, ≤ 5 µg total DNA (per well) can be eluted with ≥ 10 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
Equipment Centrifuge with microplate carriers
DNA Size Limits 50 bp to 23 kb
Sample Sources DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
DNA Purity Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
RNA Purity Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.

Clean & Concentrated DNA. DNA samples, such as the PCR products shown here, can be efficiently purified and concentrated using the DNA Clean & Concentrator™-5
Kit / Catalog # ZR-96 DNA Clean-up
Kit (D4017 and D4018)
ZR-96 DNA Clean &
Concentrator™-5
(D4023 and D4024)
Binding Plate Silicon-A™ Plate Zymo-Spin™ I-96 Plate
Style Shallow-Well Deep-Well
Height of Binding Plate 19 mm (0.75 inches) 35 mm (1.38 inches)
Binding Plate/Collection Plate Assembly 43 mm (1.69 inches) 60 mm (2.36 inches)
Binding Cap./Minimum Elution Volume 5 µg/30 µl 5 µg/15 µl

Step 1

Add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below).  Mix briefly by vortexing.

Application

DNA Binding Buffer  :  Sample

Example

Plasmid, genomic DNA (<2 kb)

2  :  1

200 µl  :  100 µl

PCR product, DNA fragment

5  :  1

500 µl  :  100 µl

ssDNA (e.g. cDNA, M13 phage1)

7  :  1

700 µl  :  100 µl

For efficient recovery of high molecular weight DNA (< 20kb to < 200kb), use the Genomic DNA Clean &  Concentrator™ (Cat. Nos. D4010, D4011).

Step 2

Transfer sample mixtures to the wells of a Zymo-Spin™ I-96 Plate2 mounted on a Collection Plate.

Step 3

Centrifuge for 5 minutes until sample mixtures have been completely filtered.  Discard the flow-through.

Step 4

Add 300 µl DNA Wash Buffer to each well of the Zymo-Spin™ I-96 Plate.  Centrifuge for 5 minutes.  Repeat wash step, but centrifuge for 15 minutes.  (Alternatively, one wash can be performed using 600 µl Wash Buffer).

Step 5

Add ≥ 10 µl DNA Elution Buffer3 or water4 directly to the column matrix in each well.  Transfer the Zymo-Spin™ I-96 Plate onto an Elution Plate and centrifuge for 3 minutes to elute the DNA. 

Ultra-pure DNA is now ready for use.

For specific notes and additional information, please see the product protocol PDF.
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“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
Rosalind P. (University of Arkansas for Medical Sciences)
“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner. The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.”
Joseph R. (Miller School of Medicine, University of Miami)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
Takashi K. (Monash University)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
Kimberly M. (University of Pennsylvania)
“It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.”
Marissa V. (Harvard Medical School)
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
Shanshan L. (UT Southwestern)
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”
Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
Tyler C.
Click here to submit your review of the ZR-96 DNA Clean & Concentrator™-5 (Deep well format).

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