About ZR-96 Oligo Clean & Concentrator™
The ZR-96 Oligo Clean & Concentrator™ provides a streamlined method for high-throughput (96-well) recovery and clean-up of DNA/RNA fragments and oligonucletides ≥16 nt from labeling (radioactive, biotin, DIG etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure.
There is no need for organic denaturants or chloroform. Instead, the kit features Fast Spin plate technology and employs a single-buffer system that allows for efficient DNA/RNA adsorption to the matrix of Zymo-Spin™ Plate. DNA/RNA is washed and concentrated into a small volume of water (≥10 µl). Purified DNA/RNA is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
|Sample Sources||Enzymatic reaction mixtures containing DNA/RNA fragments (radioactive-, biotin-, DIG-labeled) and oligonucleotides ≥16 nt.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.|
|Recovery Volume||Binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery >90%. The plate can be eluted with ≥10 µl.|
|Compatibility||single-straded (ss) and double-stranded (ds) DNA and RNA.|
|Purity||High-quality DNA/RNA (A260/A280 nm <1.8; A260/A230 nm <1.8) eluted with water is especially well suited for hybridization, sequencing, ligation, and PCR.|
|Size Limit||For oligonucletides ≥16 nt, up to 23 kb.|
Add 100 µl Oligo Binding Buffer to 50 µl sample1,2.
Add 400 µl ethanol3 (95-100%), mix briefly by pipetting and transfer the mixture to a well of the provided Zymo-Spin™ I-96 Plate4 mounted on a Collection Plate.
Note: If required, scale up volumes proportionally.
Centrifuge at 5,000 x g for 5 minutes. Discard the flow-through.
For radioactive samples: Transfer the plate onto a new collection plate then discard the plate containing the radioactive flow-through appropriately.
Add 800 µl DNA Wash Buffer to each well. Centrifuge the plate at 5,000 x g for 5 minutes and discard the flow-through. Then centrifuge at 5,000 x g for 5 minutes to ensure complete removal of the wash buffer.
Transfer the plate onto the Elution Plate. Add 25 µl water5 directly to the column matrix and centrifuge at 5,000 x g for 5 minutes to elute the oligonucleotide.
Ultra-pure oligonucleotide in water is now ready for use6.