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ZR-96 Oligo Clean & Concentrator™

Quick, high-throughput (96-well) recovery of ultra-pure DNA and RNA oligonucleotides.
Complete removal of dyes, salts, enzymes, nucleotides, and short oligos.
≥10 µl elution with zero retention Fast Spin plates.
Eluted DNA/RNA is well suited for use in hybridization, sequencing, PCR, ligation, etc.

Product Size Catalog # Price Qty
ZR-96 Oligo Clean & Concentrator (2x96) 2x96 D4062
ZR-96 Oligo Clean & Concentrator (4x96) 4x96 D4063

About ZR-96 Oligo Clean & Concentrator™

The ZR-96 Oligo Clean & Concentrator™ provides a streamlined method for high-throughput (96-well) recovery and clean-up of DNA/RNA fragments and oligonucletides ≥16 nt from labeling (radioactive, biotin, DIG etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure.

There is no need for organic denaturants or chloroform. Instead, the kit features Fast Spin plate technology and employs a single-buffer system that allows for efficient DNA/RNA adsorption to the matrix of Zymo-Spin™ Plate. DNA/RNA is washed and concentrated into a small volume of water (≥10 µl). Purified DNA/RNA is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.

Sample Sources Enzymatic reaction mixtures containing DNA/RNA fragments (radioactive-, biotin-, DIG-labeled) and oligonucleotides ≥16 nt.
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Recovery Volume Binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery >90%. The plate can be eluted with ≥10 µl.
Compatibility single-straded (ss) and double-stranded (ds) DNA and RNA.
Purity High-quality DNA/RNA (A260/A280 nm <1.8; A260/A230 nm <1.8) eluted with water is especially well suited for hybridization, sequencing, ligation, and PCR.
Size Limit For oligonucletides ≥16 nt, up to 23 kb.

The Oligo Clean & Concentrator™ facilitates >90% recovery of ssDNA oligonucleotides (A) and efficient short oligo and nucleotide removal (B).

Step 1

Add 100 µl Oligo Binding Buffer to 50 µl sample1,2.

Step 2

Add 400 µl ethanol3 (95-100%), mix briefly by pipetting and transfer the mixture to a well of the provided Zymo-Spin™ I-96 Plate4 mounted on a Collection Plate.

Note:  If required, scale up volumes proportionally.

Step 3

Centrifuge at 5,000 x g for 5 minutes.  Discard the flow-through.

For radioactive samples:  Transfer the plate onto a new collection plate then discard the plate containing the radioactive flow-through appropriately.

Step 4

Add 800 µl DNA Wash Buffer to each well.  Centrifuge the plate at 5,000 x g for 5 minutes and discard the flow-through.  Then centrifuge at 5,000 x g for 5 minutes to ensure complete removal of the wash buffer.

Step 5

Transfer the plate onto the Elution Plate.  Add 25 µl water5 directly to the column matrix and centrifuge at 5,000 x g for 5 minutes to elute the oligonucleotide.

Ultra-pure oligonucleotide in water is now ready for use6.

For specific notes and additional information, please see the product protocol PDF.
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