About ZR-96 Zymoclean™ Gel DNA Recovery Kit
The ZR-96 Zymoclean™ Gel DNA Recovery Kit provides for the rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. The product features Fast-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery kits are perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
|Format||96-well Plate (deep well)|
|DNA Recovery||Typically, up to 5 µg total DNA/well can be eluted with ≥ 15 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
|Processing Time||20 min|
|Equipment||Centrifuge w/ microplate carriers|
|DNA Size Limits||50 bp to 23 kb|
|Sample Sources||DNA excised from TAE/TBE-buffered agarose gels.|
|DNA Purity||High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, andrestriction endonuclease digestions.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.
DNA sequencing chromoatogram of a PCR product recovered using the Zymoclean™ Gel DNA Recovery Kit. DNA was recovered from a 2% (w/v) agarose gel and used directly for sequencing.
Excise the DNA fragment from the agarose gel using a razor blade, scalpel or other device and transfer it into a well of the provided Collection Plate.
Note: The amount of agarose excised from the gel should be as small as possible and should not exceed 150 µl (150 mg) per well.
|Step 2||Add 3 volumes of ADB to each volume of agarose excised from the gel (e.g. for 100 µl (mg) of agarose gel slice add 300 µl of ADB).|
Incubate at 37-55 °C for 5-10 minutes until the gel slice is completely dissolved.
For DNA fragments > 8 kb, following the incubation step, add one additional volume (equal to that of the gel slice) of water to the mixture for better DNA recovery (e.g. 100 µl agarose, 300 µl ADB and 100 µl water).
|Step 4||Transfer the melted agarose solutions to the wells of the Zymo-Spin™ I-96 Plate on the empty Collection Plate used in Step 1 (above).|
|Step 5||Centrifuge for 5 minutes until the sample mixtures have been completely filtered. Discard the flow-through in the Collection Plate.|
|Step 6||Add 300 µl of DNA Wash Buffer to each well of the Zymo-Spin™ I-96 Plate. Centrifuge for 5 minutes. Repeat the wash step, but centrifuge for 15 minutes.|
Add ≥15 µl DNA Elution Buffer or water directly to the column matrix in each well. Transfer the Zymo-Spin™ I-96 Plate onto an Elution Plate and centrifuge for 3 minutes to elute the DNA.
Ultra-pure DNA is now ready for use.