ssDNA/RNA Clean & Concentrator™

Quick (10 minute) method for separating, cleaning, and concentrating single stranded DNA or RNA.
Ideal for non-enzymatic elimination of genomic DNA from short transcripts, probes, primers, etc.
Fast-Spin column technology allows for elution into minimal volumes (≥ 6 μl).

Product Size Catalog # Price Qty
ssDNA/RNA Clean & Concentrator™ 20 Preps. D7010
$89.00
ssDNA/RNA Clean & Concentrator™ 50 Preps. D7011
$179.00

About ssDNA/RNA Clean & Concentrator™

The ssDNA/RNA Clean & Concentrator™ provides a simple and reliable method for the rapid separation, clean-up, and concentration of up to ~5 µg (per prep.) of single stranded DNA and/or RNA from double stranded species (e.g., genomic DNA). This simple 10 minutes procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. Single stranded DNA or RNA ≥ 17 to 200 nucleotides (e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highly concentrated, purified DNA/RNA that is suitable for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.


Format Spin Column
Processing Time 10 min
Equipment Microcentrifuge
Sample Sources Mixtures of double and single stranded DNA and RNA species with single stranded fragments 17 to 200 nucleotides (e.g., short transcripts, probes, primers).
Elution Volume ≥ 6µl
Storage ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage.
Purity High quality DNA/RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream manipulations.
Recovery Up to 5 μg ssDNA/RNA

Step 1

Add 2 volumes of DNA/RNA Binding Buffer to the sample1 (e.g., 200 μl buffer to 100 μl sample) and mix well.

Step 2

Transfer the mixture to the Zymo-Spin™ IIC Column in a Collection Tube and centrifuge at ≥12,000 x g for 1 minute.  Save the flow-through!

Step 3

Add 1 volume ethanol (95-100%) to the flow-through in the Collection Tube (e.g., 300 μl ethanol to 300 μl flow-through), and mix well by pipetting.

Step 4

Transfer the mixture to the Zymo-Spin™ IC Column in a Collection Tube and centrifuge at ≥12,000 x g for 1 minute.  Discard the flow-through.

Step 5

Add 400 µl DNA/RNA Prep Buffer to the column and centrifuge at ≥12,000 x g for 1 minute.  Discard the flow-through.

Step 6

Add 700 µl DNA/RNA Wash Buffer to the column and centrifuge at ≥12,000 x g for 30 seconds.  Discard the flow-through and repeat Step 6 with 400 µl DNA/RNA Wash Buffer.

Step 7

Centrifuge the Zymo-Spin™ IC Column in an emptied Collection Tube at ≥12,000 x g for 2 minutes.  Transfer the column into an RNase-Free Tube

Step 8

Add ≥6 µl DNase/RNase-Free Water2 directly to the column matrix and let stand for 1 minute at room temperature.  Centrifuge at 10,000 x g for 30 seconds.  The eluted DNA/RNA can be used immediately or stored at or below -20°C.

For specific notes and additional information, please see the product protocol PDF.
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