About ZymoTaq™ DNA Polymerase
ZymoTaq™ DNA Polymerase is a hot-start polymerase that is ideal for amplification of bisulfite-converted DNA. Because it is a heat-activated, thermostable DNA polymerase, ZymoTaq™ reduces primer dimer and nonspecific product formation, whereas conventional polymerases typically exhibit these problems with bisulfite-converted DNA templates. In addition to the amplification of bisulfite-treated DNA for methylation detection, ZymoTaq™ DNA polymerase can also be used for conventional PCR, and real time PCR. The enzyme also has 3-terminal transferase activity, making it ideal for use in TA-cloning by the addition of "A" overhangs to amplified DNA.
|Format||Provided as a PreMix (E2003, E2004) or as a component of a set (E2001, E2002).|
|Concentration||Reaction conditions at 1X (50 µl total volume) contain 2 U of ZymoTaq™ DNA Polymerase. For the ZymoTaq™ PreMix (2X), the enzyme is at 4 U/50 µl.|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at -80°C.|
|Unit Definition||One unit (1U) is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.|
|Optimum pH and Temperature||72°C|
|Activity||5' - 3' DNA polymerization|
The ZymoTaq™ PreMix was specifically used to amplify bisulfite-treated DNA with no reports of inefficient or nonspecific amplification in a study of dynamic DNA methylation expression levels during different development stages. Results showed that timing of variations in methylation patterns were specific for genes related to growth.