About DNA Degradase™
DNA Degradase™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
|Format||Provided in solution (10 units/µl) w/ 10X Reaction Buffer|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be ≤ -70°C.|
|Unit Definition||One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C.|
|Reaction Conditions||DNA Degradase™ in 1X DNA Degradase™ Reaction Buffer. Incubate reaction mixtures at 37°C for ≥1 hour.|
|Inactivation||Heat-inactivate at 70°C for 20 minutes.|
|Standard Reaction Setup||The setup (below) is an example of a typical DNA Degradase™ reaction in a 25 µl final volume.
DNA Degradase™ efficiently degrades DNA: 1 µg, 5 µg, and 10 µg of λ DNA incubated with 1 µl (10 U) of DNA Degradase™ in 25 µl reaction volume and incubated at 37°C for 1 hour.