About Micrococcal Nuclease
Micrococcal Nuclease cleaves single stranded and double stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono and oligonucleotides with 3' phosphates.
|Format||Typical buffer consists of 20mM Tris-HCl, (pH 8.8), 1mM CaCl2. CaCl2 is essential for activity.|
|Enzyme Commission Number||(EC 18.104.22.168)|
|Unit Definition||One unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.|
|Enzyme Concentration||0.1 U/µl|
|Enzyme Inactivation||EDTA or EGTA in molar excess of CaCl2|
|Optimum Reaction Temperature||37°C|
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