Credit Orders: Currently, online ordering is not available via credit card (PO can still be processed).
Please call 888-882-9682 for assistance. We apologize for the inconvenience; use code NEWWEB10 for a 10% discount.

Micrococcal Nuclease


Product Size Catalog # Price Qty
Micrococcal Nuclease n/a D5220-1
$24.00

About Micrococcal Nuclease

Micrococcal Nuclease cleaves single stranded and double stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono and oligonucleotides with 3' phosphates.


Format Typical buffer consists of 20mM Tris-HCl, (pH 8.8), 1mM CaCl2. CaCl2 is essential for activity.
Enzyme Commission Number (EC 3.1.31.1)
Unit Definition One unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.
Enzyme Concentration 0.1 U/µl
Enzyme Inactivation EDTA or EGTA in molar excess of CaCl2
Optimum Reaction Temperature 37°C

Submit your Citation
GETTING PUBLISHED? Take some time to celebrate and enjoy the little things in life!

Getting published is not only hard work, it takes enormous amounts of time and dedication. Zymo Research would like to celebrate your achievement! Let us know when you have cited any of our products in your recent publication and get a $20 Amazon gift code.

Click here to submit your review of the Micrococcal Nuclease.

Call Us

1.888.882.9682

We're here Mon - Fri 8am - 5pm PST

Send an Email

info@zymoresearch.com

We'll reply within 24 hours