About Proteinase K
Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.
|Format||Lyophilized enzyme provided w/ storage buffer|
|Enzyme Commission Number||(EC 18.104.22.168)|
|Unit Definition||One unit (1 U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.|
|pH and Temperature Range||pH 4.0 to 12.0 (8.0 is optimum), 25 to 65°C|
|Specific Activity||>30 units/mg protein|