About Quick-gDNA™ Blood MicroPrep
Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to Quick-gDNA™ MicroPrep (D3020/D3021) for the replacement product.The Quick-gDNA™ Blood MicroPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.
|DNA Recovery||Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.|
|Processing Time||15 min|
|DNA Size Limits||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Sample Sources||Sources: blood, plasma, or serum from humans, mice, rats, etc.|
|DNA Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.|
Transfer the mixture to a Zymo-Spin™ IC Column2 in a Collection Tube. Centrifuge at 10,000 x g for one minute. Discard the Collection Tube with the flow through.
Transfer the Zymo-Spin™ Column to a new Collection Tube. Add 200 µl of DNA Pre-Wash Buffer to the spin column. Centrifuge at 10,000 x g for one minute.
Add 500 µl of g-DNA Wash Buffer to the spin column. Centrifuge at 10,000 x g for one minute.
Transfer the spin column to a clean microcentrifuge tube. Add ≥10 µl DNA Elution Buffer or water to the spin column3. Incubate 2-5 minutes at room temperature and then centrifuge at top speed for 30 seconds to elute the DNA. The eluted DNA can be used immediately for molecular based applications or stored ≤-20ºC for future use.