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Quick-gDNA™ Blood MicroPrep

Quick purification of high quality DNA from whole blood, plasma, and serum in less than 15 minutes using innovative Fast-Spin column technology.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Product Size Catalog # Price Qty
Quick-gDNA™ Blood MicroPrep 50 Preps. D3070
Quick-gDNA™ Blood MicroPrep 200 Preps. D3071

About Quick-gDNA™ Blood MicroPrep

Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to Quick-gDNA™ MicroPrep (D3020/D3021) for the replacement product.

The Quick-gDNA™ Blood MicroPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.

Format Spin Column
DNA Recovery Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.
Processing Time 15 min
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Sources: blood, plasma, or serum from humans, mice, rats, etc.
DNA Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Equipment Needed Microcentrifuge

Step 1

Add 200 µl of Genomic Lysis Buffer to 50 µl of blood, serum, or plasma (4:1).  Mix completely by vortexing 4-6 seconds, then let stand 5-10 minutes at room temperature.1

Note:  Add 200 µl Genomic Lysis Buffer to all samples <50 µl.  For samples larger than 50 µl, add a proportional amount (4:1) of Genomic Lysis Buffer (e.g., Add 800 µl Genomic Lysis Buffer to 200 µl blood).

Step 2

Transfer the mixture to a Zymo-Spin™ IC Column2 in a Collection Tube.  Centrifuge at 10,000 x g for one minute.  Discard the Collection Tube with the flow through.

Step 3

Transfer the Zymo-Spin™ Column to a new Collection Tube.  Add 200 µl of DNA Pre-Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.

Step 4

Add 500 µl of g-DNA Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.

Step 5

Transfer the spin column to a clean microcentrifuge tube. Add ≥10 µl DNA Elution Buffer or water to the spin column3.  Incubate 2-5 minutes at room temperature and then centrifuge at top speed for 30 seconds to elute the DNA. The eluted DNA can be used immediately for molecular based applications or stored ≤-20ºC for future use.

For specific notes and additional information, please see the product protocol PDF.
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