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ZR-96 Quick-gDNA™ Blood

Quick, high throughput purification of high quality DNA from whole blood, plasma, and serum in less than 25 minutes using innovative Fast-Spin 96-well plate technology.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Product Size Catalog # Price Qty
ZR-96 Quick-gDNA™ Blood 2 x 96 Preps. D3075
ZR-96 Quick-gDNA™ Blood 4 x 96 Preps. D3076
ZR-96 Quick-gDNA™ Blood 10 x 96 Preps. D3077

About ZR-96 Quick-gDNA™ Blood

Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to ZR-96 Quick-gDNA™ (D3010/D3011/D3012) for the replacement product.

The ZR-96 Quick-gDNA™ Blood is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.

Format 96-well
DNA Recovery Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.
Processing Time 25 min
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Whole blood, plasma, or serum from humans, mice, rats, etc.
DNA Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Equipment Needed Microcentrifuge, vortex, centrifuge w/ microplate carriers

Step 1

Add 200 µl of Genomic Lysis Buffer to 50 µl of blood, serum, or plasma (4:1).  Mix completely by vortexing 4-6 seconds, then let stand 5-10 minutes at room temperature.1

Note:  Add 200 µl Genomic Lysis Buffer to all samples <50 µl.  For samples larger than 50 µl, add a proportional amount (4:1) of Genomic Lysis Buffer (e.g., Add 800 µl Genomic Lysis Buffer to 200 µl blood).

Step 2

Transfer the mixtures to the wells of a Silicon-A™ Plate2 on a Collection Plate.  Centrifuge at ≥ 2,500 x g (5,000 x g max.) for 5 minutes.

Step 3

Add 200 µl DNA Pre-Wash Buffer to each well and centrifuge at ≥ 2,500 x g for 5 minutes.  Discard the flow through.

Step 4

Add 300 µl of g-DNA Wash Buffer to each well and centrifuge at ≥ 2,500 x g for 5 minutes.

Step 5

Transfer the Silicon-A™ Plate onto an Elution Plate. Add ≥30 µl DNA Elution Buffer or water to each well.  Incubate 2-5 minutes at room temperature, then centrifuge at ≥ 2,500 x g for 5 minutes to elute the DNA. The eluted DNA can be used immediately for molecular based applications or stored ≤-20ºC for future use.

For specific notes and additional information, please see the product protocol PDF.
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