About ZR-96 Quick-gDNA™ Blood
Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to ZR-96 Quick-gDNA™ (D3010/D3011/D3012) for the replacement product.The ZR-96 Quick-gDNA™ Blood is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.
|DNA Recovery||Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.|
|Processing Time||25 min|
|DNA Size Limits||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Sample Sources||Whole blood, plasma, or serum from humans, mice, rats, etc.|
|DNA Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.|
|Equipment Needed||Microcentrifuge, vortex, centrifuge w/ microplate carriers|
Transfer the mixtures to the wells of a Silicon-A™ Plate2 on a Collection Plate. Centrifuge at ≥ 2,500 x g (5,000 x g max.) for 5 minutes.
Add 200 µl DNA Pre-Wash Buffer to each well and centrifuge at ≥ 2,500 x g for 5 minutes. Discard the flow through.
Add 300 µl of g-DNA Wash Buffer to each well and centrifuge at ≥ 2,500 x g for 5 minutes.