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Quick-DNA™ 96 Plus Kit

Easy and reliable high-throughput DNA purification from any sample source (whole blood, biological fluids, cultured/monolayer cells, solid tissues, etc.).
Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA-sequencing, arrays, and methylation analysis.

Product Size Catalog # Price Qty
Quick-DNA™ 96 Plus Kit 2x96 Wells D4070
$530.00
Quick-DNA™ 96 Plus Kit 4x96 Wells D4071
$989.00

About Quick-DNA™ 96 Plus Kit

Note: The Quick-DNA™ Universal 96 Kit (D4070/D4071) has been renamed to Quick-DNA™ 96 Plus Kit; all components and protocols are the same and have not been modified.

The Quick-DNA™ 96 Plus Kit is the easiest method for high-throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-SpinTM technologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 15 µl.  Zymo-Spin Plates ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis. 


Equipment Water bath or heat block (55oC), microcentrifuge, and vortex.
Sample Sources See full protocol for details
DNA Purity High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0).
Elution Volume DNA can be eluted into as little as 15 µl DNA Elution Buffer or water.
DNA Yield The DNA binding capacity of each well is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
DNA Size Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
DNA Applications DNA isolated using the Quick-DNA™ 96 Plus Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications.
DNA Types The Quick-DNA™ 96 Plus Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. Not recommended for small cell-free DNA isolation from urine, serum, and plasma.
Workflow Overview Utilize a Proteinase K Digestion and Zymo-Spin™ Column for effective recovery of DNA. See page 5 for more information.

High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ 96 Plus Kit. DNA purified using the Quick-DNA™ 96 Plus Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).

 

HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ 96 Plus Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ 96 Plus Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.

Resuspend ≤ 1 x 106 mammalian or insect cell pellets using 50 µl DNA Elution Buffer or an isotonic buffer (e.g. PBS).

Overnight Proteinase K digestions at 55ºC are possible without affecting the integrity of the DNA


Biologicial Fluids & Cells

Solid Tissues

1. Add up to 50 µl1 sample to each well of a Deep-Well Block and add:

  • 50 µl BioFluid & Cell Buffer (Red)
  • 5 µl Proteinase K

2. Mix thoroughly, seal with film, and then incubate at 55°C for 20 minutes.

3. Add 1 volume Genomic Binding Buffer to each well and mix thoroughly by repeated pipetting.

1. To tissue samples (≤ 5 mg) in a Deep-Well Block, add to each well a solution of:

  • 45 µl Water

  • 45 µl Solid Tissue Buffer (Blue)
  • 10 µl Proteinase K

2. Mix thoroughly, seal with film, and then incubate the tube at 55°C for 1-3 hours or until tissue clarifies. Mix thoroughly before proceeding.

3. Add 2 volumes Genomic Binding Buffer to each well. Mix thoroughly by repeated pipetting.

  1. Transfer the lysates to the wells of the Zymo-Spin™ I-96-XL Plate on a Collection Tube. Centrifuge at ≥ 3,500 x g for 5 minutes3. Discard the flow-through.
  2. Add 200 µl DNA Pre-Wash Buffer to each well. Centrifuge at ≥ 3,500 x g for 5 minutes. Discard the flow-through.
  3. Add 500 µl g-DNA Wash Buffer to each well. Centrifuge at ≥ 3,500 x g for 5 minutes. Discard the flow-through.
  4. Add 200 µl g-DNA Wash Buffer to each well. Centrifuge at ≥ 3,500 x g for 5 minutes. Discard the flow-through.
  5. Transfer the 96-well plate to an Elution Plate. Add ≥ 15 µl DNA Elution Buffer or water4 directly on the matrix. Incubate 3 minutes at room temperature, then centrifuge at ≥ 3,500 x g for 5 minutes to elute the DNA5. The eluted DNA can be used immediately for molecular based applications or stored ≤ -20°C for future use.
For specific notes and additional information, please see the product protocol PDF.
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