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Quick-DNA™ Microprep Kit

Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 15 minutes using innovative Zymo-Spin™ column technology.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Product Size Catalog # Price Qty
Quick-DNA™ Microprep Kit 50 Preps. D3020
Quick-DNA™ Microprep Kit 200 Preps. D3021

About Quick-DNA™ Microprep Kit

The Quick-DNA™ Microprep Kit is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources.  This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples. 

For processing, simply add the specially formulated Genomic Lysis Buffer to a sample, vortex, and transfer the mixture to the supplied Zymo-Spin™ Column.  There is no need for organic denaturants or Proteinase K digestion because of the unique lysis buffer system.  The product features Zymo-Spin technology to yield high-quality, purified DNA in just minutes (see below).  PCR inhibitors are effectively removed during the purification process.  DNA purified using the Quick-DNA™ Microprep Kit is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.  

DNA Recovery Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues.
Equipment microcentrifuge, vortex
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed.
DNA Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Workflow Overview Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.

DNA isolated from porcine whole blood using the Quick-DNA™ Microprep Kit. Equivalent amounts (100 µl) of blood were processed without Proteinase K using the Quick-DNA™ Microprep Kit in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research).

Step 1

In a 1.5 ml microcentrifuge tube, add up to 50 µl (max.) of blood, serum, or plasma to 200 µl of Genomic Lysis Buffer.  Mix completely by vortexing 4-6 seconds, then let stand 5-10 minutes at room temperature.

Step 2

Transfer the mixture to a Zymo-Spin™ IC Column in a Collection Tube.  Centrifuge at 10,000 x g for one minute.  Discard the Collection Tube with the flow through.

Step 3

Transfer the Zymo-Spin™ IC Column to a new Collection Tube.  Add 200 µl of DNA Pre-Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.

Step 4

Add 500 µl of g-DNA Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.   

Step 5

Transfer the spin column to a clean microcentrifuge tube. Add ≥10 µl DNA Elution Buffer or water to the spin column.  Incubate 2-5 minutes at room temperature and then centrifuge at top speed for 30 seconds to elute the DNA. The eluted DNA can be used immediately for molecular based applications or stored ≤-20ºC for future use. 

For specific notes and additional information, please see the product protocol PDF.
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“This kit did a good job of prepping clean genomic DNA.”
Tara N. (United States Agricultural Research Service)
“It was easy to work with, protocol easy to follow”
Tinatin T.
Click here to submit your review of the Quick-DNA™ Microprep Kit.

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