Quick-DNA™ Miniprep Kit

Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 15 minutes using innovative Zymo-Spin™ column technology.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Product Size Catalog # Price Qty
Quick-gDNA™ MiniPrep - Uncapped column 50 Preps. D3006
$83.00
Quick-DNA™ Miniprep Kit - Capped column 50 Preps. D3024
$98.00
Quick-DNA™ Miniprep Kit - Capped column 200 Preps. D3025
$320.00

About Quick-DNA™ Miniprep Kit

Note: The product cat. no. D3006 and D3007 (uncapped column version) will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to D3024 or D3025 (capped column version) for the direct replacement product.

The Quick-DNA™ Miniprep Kit is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources.  This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples. 

For processing, simply add the specially formulated Genomic Lysis Buffer to a sample, vortex, and transfer the mixture to the supplied Zymo-Spin™ Column.  There is no need for organic denaturants or Proteinase K digestion because of the unique lysis buffer system.  The product features Zymo-Spin technology to yield high-quality, purified DNA in just minutes (see below).  PCR inhibitors are effectively removed during the purification process.  DNA purified using the Quick-DNA™ Miniprep Kit is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.  


DNA Recovery Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues.
Equipment microcentrifuge, vortex
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed.
DNA Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Workflow Overview Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.

DNA isolated from porcine whole blood using the Quick-DNA™ Miniprep Kit. Equivalent amounts (100 µl) of blood were processed without Proteinase K using the Quick-gDNA™ MiniPrep in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research).

Step 1

Add 400 µl of Genomic Lysis Buffer to 100 µl of blood, serum, or plasma (4:1).  Mix completely by vortexing 4-6 seconds, then let stand 5-10 minutes at room temperature.

Note:  Add 200 µl Genomic Lysis Buffer to all samples <50 µl.  For samples larger than 50 µl, add a proportional amount (4:1) of Genomic Lysis Buffer (e.g., Add 800 µl Genomic Lysis Buffer to 200 µl blood).    

Step 2

Transfer the mixture to a Zymo-Spin™ Column in a Collection Tube.  Centrifuge at 10,000 x g for one minute.  Discard the Collection Tube with the flow through.

Step 3

Transfer the Zymo-Spin™ Column to a new Collection Tube.  Add 200 µl of DNA Pre-Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.

Step 4

Add 500 µl of g-DNA Wash Buffer to the spin column.  Centrifuge at 10,000 x g for one minute.   

Step 5

Transfer the spin column to a clean microcentrifuge tube. Add ≥50 µl DNA Elution Buffer or water to the spin column.  Incubate 2-5 minutes at room temperature and then centrifuge at top speed for 30 seconds to elute the DNA. The eluted DNA can be used immediately for molecular based applications or stored ≤-20ºC for future use. 

For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

The Quick-gDNA MiniPrep enabled the recovery of high quality DNA from blood pathogens for whole genome analysis of bacterium that cannot be cultured in vitro. The sequenced genome provided clues to the mechanism of pathogenicity.
The Quick-gDNA MiniPrep kit was used to extract inhibitor-free DNA from the blood of pigs infected with Mycoplasma suis. The purified DNA was used in the development of a qPCR assay that is highly sensitive and specific for M. suis detection and quantification.

"This product was amazing! I've used the same type of kit (quick DNA extract) from Sigma and this was far more superior. I used the same amount of postnatal tissue as I would have for the Sigma kit, however the yield I obtained from Zymo was quite astounding considering the time of tissue digestion. Secondly, the gDNA was much 'cleaner' upon measurement with our NanoDrop! "
Stephen C. (Johns Hopkins University School of Medicine)
“This kit did a good job of prepping clean genomic DNA.”
Tara N. (United States Agricultural Research Service)
“It was easy to work with, protocol easy to follow”
Tinatin T.
Click here to submit your review of the Quick-DNA™ Miniprep Kit.

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