About ZR-96 Genomic DNA Clean & Concentrator™-5
The ZR-96 Genomic DNA Clean & Concentrator™-5 (DCC™) is for high throughput recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Bufferto a sample and then transfer the mixture to the supplied Zymo-Spin™ I-96-XL Plate. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
|DNA Recovery||Typically, up to 5 µg total DNA per column can be eluted into as ≥15 µl of low salt DNA Elution Bufferor water.|
|DNA Size Limits||Capable of purifying small DNA fragments >50 bp and large sized DNAs up to 200 kb.|
|Sample Sources||DNA from impure preparations of genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and whole genome amplified (wga) DNA. Can also be used for the purification of low molecular weight DNA (50 bp to 10 kb) from PCR, endonuclease digestion, post-RT cDNA synthesis, etc.|
|DNA Purity||High-quality (A(260/280) ≥1.8) high molecular weight DNA ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS.|