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Quick-DNA™ Microprep Plus Kit

Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
Zymo-Spin™ Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.

Product Size Catalog # Price Qty
Quick-DNA™ MicroPrep Plus Kit 50 Preps D4074

About Quick-DNA™ Microprep Plus Kit

The Quick-DNA Microprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 10 µl.  Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.

Equipment Water bath or heat block (55˚C), microcentrifuge, and vortex.
Sample Sources See pages 2 and 3.
DNA Purity High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0).
Elution Volume DNA can be eluted into as little as 10 µl DNA Elution Buffer or water.
DNA Yield The DNA binding capacity of each column is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
DNA Size Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
DNA Applications DNA isolated using the Quick-DNA™ Microprep Plus Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications.
DNA Types The Quick-DNA™ Microprep Plus Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. from biological fluids, cells, or tissues. Not recommended for small cell-free DNA isolation from urine, serum, and plasma.
Workflow Overview Utilizes a Proteinase K Digestion and Zymo-Spin™ Technology for effective recovery of DNA. See page 5 for more information.

High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ Miniprep Plus Kit. DNA purified using the Quick-DNA™ Miniprep Plus Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA™ Miniprep Plus Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed. HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ Miniprep Plus Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ Miniprep Plus Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.
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