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Quick-DNA™ Fecal/Soil Microbe 96 Kit

Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, cattle, etc.
Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Product Size Catalog # Price Qty
Quick-DNA™ Fecal/Soil Microbe 96 Kit 2x96 Preps D6011

About Quick-DNA™ Fecal/Soil Microbe 96 Kit

The ZR-96 Fecal DNA Kit™ (D6011) has been updated to Quick-DNA™ Fecal/Soil Microbe 96 Kit; all components and protocols are the same, catalog numbers and names of components may have been modified but original buffer recipe remains the same.

The Quick-DNA™ Fecal/Soil Microbe 96 Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin plate technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Format 96-well Plate
DNA Recovery Typically, up to 5 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample.
Processing Time 50 min
Equipment Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer.
DNA Size Limits Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 kb "“ 35 kb. If present, parasitic and viral DNA will also be recovered.
Sample Sources Bacterial, protist, as well as host DNA can be effectively isolated from a ≤80 mg sample of mammalian feces.
DNA Purity High quality, inhibitor-free DNA is eluted with DNA Elution Buffer suitable for the amplification of bacterial, protist, and/or mammalian templates. Absorbance 260 nm/280 nm >1.8.

Comparison of DNA yields from rat feces using the Quick-DNA™ Fecal/Soil Microbe 96 Kit and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.

Step 1

Add up to 80 milligrams of fecal sample to the tubes of a ZR BashingBead™ Lysis Rack.  Add 400 µl Lysis Solution to each tube.  Cap tubes tightly to prevent leakage.

Step 2

Secure in a 96-well block/plate bead beater (e.g., 2010 GenoGrinder®) and process samples.  Optimization of processing time/speed will be necessary for complete sample lysis.

Processing times may be as little as one minute when using high-speed cell disrupters (e.g., 2000 GenoGrinder®, page 5).  See manufacturer’s literature for operating information.

Step 3

Centrifuge the ZR BashingBead™ Lysis Rack at ≥ 3,000 x g (5,000 x g max.) for 5 minutes.

Step 4

Transfer up to 250 µl supernatant to each well of a Deep-Well Block.

Step 5

Add 750 µl of Fecal DNA Binding Buffer to the supernatant in the Deep-Well Block from Step 4.  Cover completely with Cover Foil and mix thoroughly by vortexing for 2 minutes.  Centrifuge the Deep-Well Block at ≥ 3,000 x g (5,000 x g max.) for 5 minutes.

Step 6

Remove or pierce foil and transfer 500 µl of each of the mixtures from Step 5 to the wells of a Silicon-A™ Plate on a Collection Plate.  Centrifuge the assembly at ≥ 3,000 x g (5,000 x g max.) for 5 minutes.

Step 7

Discard the flow through from the Collection Plate and repeat Step 6.

Step 8

Add 200 µl DNA Pre-Wash Buffer to the wells of the Silicon-A™ Plate on the emptied Collection Plate and centrifuge the assembly at ≥ 3,000 x g for 5 minutes.

Step 9

Add 500 µl Fecal DNA Wash Buffer to the wells of the Silicon-A™ Plate on the Collection Plate and centrifuge the assembly at ≥ 3,000 x g for 5 minutes.

Step 10

Transfer the Silicon-A™ Plate onto a clean Elution Plate and add 100 µl (50 µl minimum) DNA Elution Buffer directly to the matrices in the plate. Centrifuge the assembly at ≥ 3,000 x g for 5 minutes.

Step 11

Transfer the eluted DNA from Step 10 to a prepared Silicon-A™-HRC Plate mounted onto a clean Elution Plate. Centrifuge the assembly at exactly 3,500 x g for 3 minutes. 

Eluted, ultra-pure DNA is now ready for use in your experiments, or the Elution Plate can be covered with Cover Foil for storage of the DNA.     

For specific notes and additional information, please see the product protocol PDF.
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