Xpedition™ Plant/Seed DNA MiniPrep

"Take the Lab to the Field" and to the site of sample collection with state of the art Xpedition™ Sample Prep and DNA preservation technologies.
For efficient BashingBead™ lysis and isolation of inhibitor-free, PCR-quality DNA directly from tough-to-lyse plant and seed samples.
Unique lysis/stabilization solution keeps DNA intact in lysates for storage and transport following remote sample processing with the Xpedition™ Sample Processor (XSP).

Product Size Catalog # Price Qty
Xpedition™ Plant/Seed DNA MiniPrep 50 Preps. D6221
$292.00

About Xpedition™ Plant/Seed DNA MiniPrep

Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to ZR Plant/Seed DNA MiniPrep (D6020) for the replacement product; the Xpedition Stabilization/Lysis Solution (D6202-1-40) can be purchased separately.

Degradation and contamination of biological samples have been obstacles to scientific study, and may be particularly problematic in highly sensitive molecular-analysis techniques (e.g., PCR of low copy DNA). Use of cryogenic freezing methods for environmental/forensic sample preservation may often be too impractical to be employed. The Xpedition™ Plant/Seed DNA MiniPrep, when used with a portable bead beating device [i.e., Xpedition™ Sample Processor (XSP), pg. 6], allows the researcher/investigator to "Take the Lab to the Field" and to the site of sample collection. The Xpedition™ Plant/Seed DNA MiniPrep is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. Samples from remote locations can be processed at the site of collection using the provided ZR BashingBead™ Lysis Tubes and a XSP (available separately, Cat. No. S6020, pg. 6). Sample processing occurs in a unique lysis/stabilization solution that effectively preserves DNA in plant lysates for subsequent storage and transport. Later, when convenient with the researcher, the kit's Fast-Spin column technologies are used for the isolation of polyphenol, lipid, polysaccharide-free DNA from the lysates. Eluted DNA is ideal for PCR for genotypic analysis including metagenomics, barcoding, phylogenetics, forensics, etc. An overview of the procedure is shown below.


Format Bead Beating, Spin Column
DNA Recovery Typically, up to 25 µg total DNA is eluted into 50-100 µl (25 µl minimum) DNA Elution Buffer per sample.
Equipment Microcentrifuge, vortex, cell disrupter/pulverizer (optional)
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Up to 150 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.
DNA Purity High quality, inhibitor-free DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. A260/A280 < 1.8
DNA Yield Typically 20-80 ng DNA/mg plant material.

Step 1

Add up to 150 mg of finely cut plant or seed sample to a ZR BashingBead™ Lysis Tube.  Add 750 µl Xpedition™ Lysis/Stabilization Solution to the tube.

Note: The Xpedition™ Lysis/Stabilization Solution can be added to the ZR BashingBead™ Lysis Tube ahead of time, prior to sample addition

Step 2

Secure in a bead beater fitted with a 2 ml tube holder assembly (e.g., Xpedition™ Sample Processor for processing at the site of sample collection) and process for a minimum of 30 seconds.  Store/transport samples.  Proceed to Step 3 when convenient.

Step 3

Centrifuge the ZR BashingBead™ Lysis Tube in a microcentrifuge at ≥10,000 x g for 1 minute.

Step 4

Transfer up to 400 µl supernatant to a Zymo-Spin™ IV Spin Filter (orange top) in a Collection Tube and centrifuge at 7,000 x g (~7,000 rpm) for 1 minute.

Step 5

Add 1,200 µl of Plant/Seed DNA Binding Buffer to the filtrate in the Collection Tube from Step 4 and mix.

Step 6

Transfer 800 µl of the mixture from Step 5 to a Zymo-Spin™ IIC Column in a Collection Tube and centrifuge at 10,000 x g for 1 minute.  

Step 7

Discard the flow through from the Collection Tube and repeat Step 6.

Step 8

Add 200 µl DNA Pre-Wash Buffer to the Zymo-Spin™ IIC Column in a new Collection Tube and centrifuge at 10,000 x g for 1 minute.

Step 9

Add 500 µl Plant/Seed DNA Wash Buffer to the Zymo-Spin™ IIC Column and centrifuge at 10,000 x g for 1 minute.  

Step 10

Transfer the Zymo-Spin™ IIC Column to a clean 1.5 ml microcentrifuge tube and add 50-100 µl (25 µl minimum) DNA Elution Buffer directly to the column matrix.  Centrifuge at 10,000 x g for 30 seconds to elute the DNA.

Step 11

 Transfer the eluted DNA from Step 10 to a prepared Zymo-Spin™ IV-HRC Spin Filter (green top) (see above) in a clean 1.5 ml microcentrifuge tube and centrifuge at exactly 8,000 x g for 1 minute.  The filtered DNA is now suitable for PCR and other downstream applications.     

For specific notes and additional information, please see the product protocol PDF.
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