ZymoPURE™ Plasmid Maxiprep Kit

The fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra-pure endotoxin-free plasmid DNA.
Routinely recover ≥ 1 µg/µl plasmid DNA directly from a microcentrifuge column that is ideal for transfection and other sensitive downstream applications.

Product Size Catalog # Price Qty
ZymoPURE™ Plasmid Maxiprep Kit 10 Preps D4202
$206.00
ZymoPURE™ Plasmid Maxiprep Kit 20 Preps D4203
$398.00

About ZymoPURE™ Plasmid Maxiprep Kit

ZymoPURE Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

As an added convenience, the ZymoPURE Plasmid Maxiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.

*Patent Pending


Processing Time 18 min
Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
DNA Purity Eluted DNA is ultrapure and well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
Recovery Volume ≥ 200 µl of ZymoPURE Elution Buffer or DNase free water
Recommended Sample Volume 150 ml E. coli Culture

 

Yield, concentration and transfection efficiency for plasmid DNA isolated using the ZymoPURE Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo Luciferase Assay System and Veritas Microplate Luminometer. Shown are means ± SEM of 8 transfections.

 

Plasmid DNA yield and concentration from the ZymoPure Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

  1. Add 14 ml of ZymoPURE P1 (Red)to the bacterial cell pellet and resuspend completely by vortexing or pipetting.
  2. Add 14 ml of ZymoPURE P2 (Green) and immediately mix by gently inverting the tube 6 times. Do not vortex! Let sit at room temperature for 2-3 minutes. Cells are completely lysed when the solution appears clear, purple, and viscous.
  3. Add 14 ml of ZymoPURE P3 (Yellow) and mix gently but thoroughly by inversion. Do not vortex! The sample will turn yellow when the neutralization is complete and a yellowish precipitate will form.
  4. Ensure the plug is attached to the Luer Lock at the bottom of the ZymoPURE Syringe Filter. Place the syringe filter upright in a tube rack and load the lysate into the ZymoPURESyringe Filter and wait 5 - 8 minutes for the precipitate to float to the top.
  5. Remove the Luer Lock plug from the bottom of the syringe and place it into a clean 50 ml conical tube. Place the plunger in the syringe and push the solution through the ZymoPURE Syringe Filter to clear the debris. Save the cleared lysate!
  6. Add 14 ml ZymoPURE Binding Buffer to the cleared lysate from step 6 and mix thoroughly by inverting the capped tube 10 times.

Vacuum Protocol

The vacuum pump should be a single
or double-staged unit capable of
producing up to 400 mm Hg pressure.

Centrifugation protocol

A swinging bucket rotor is required for centrifugation.

8. Ensure the connections of the Zymo-Spin V-P Column Assembly are finger-tight and place onto a vacuum manifold.

9. Add the entire mixture from step 7 into the Zymo-Spin V-P Column Assembly, and then turn on the vacuum until all of the liquid has passed completely through the column.

10. Remove and discard the 50 ml Reservoir from the top of the Zymo-Spin V-P Column Assembly.

8. Remove the 50 ml Reservoir from the top of the Zymo-Spin V-P Column Assembly. Ensure the connection between the 15 ml Conical Reservoir and Zymo-Spin V-P column is finger-tight and place the assembly into a 50 ml conical tube.

9. Add 14 ml of the mixture from step 7 into the 15 ml Conical Reservoir/Zymo-Spin™ V-P Column assembly, and centrifuge at 500 x g for 2 minutes.

10. Empty the 50 ml conical tube and repeat step 9 until the entire sample has passed through the column.

Note: Steps 11-12 can also be completed using a microcentrifuge instead of the vacuum manifold (see full instruction manual).

  1. With the vacuum off, add 5 ml of ZymoPUREWash 1 to the Zymo-Spin V-P Column.Turn on the vacuum until all of the liquid has passed completely through the column.
  2. With the vacuum off, add 5 ml of ZymoPUREWash 2 to the Zymo-Spin V-P Column. Turn on the vacuum until all of the liquid has passed completely through the column. Repeat this wash step.
  3. Place the Zymo-SpinV-P Column in a Collection Tube and transfer to a microcentrifuge. Centrifuge at ≥10,000 x g for 1 minute in order to remove any residual wash buffer.
  4. Transfer the Zymo-Spin V-P Column into a clean 1.5 ml tube and add 400 µl of ZymoPURE Elution Buffer directly to the column matrix. Incubate at room temperature for 2 minutes, and then centrifuge at ≥10,000 x g for 1 minute in a microcentrifuge.
For specific notes and additional information, please see the product protocol PDF.
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“I believe that the Maxi kit is the same quality that I have come to expect with other zymo products”
C. H., Ann and Robert H. Lurie Children’s Hospital of Chicago
“I used plasmid for transfection and obtained a very high expression of recombinant protein. The plasmid is well tolerated by insect cell cultures and transfected efficiently, comparable to caesium chloride gradient prep.”
M. A., University of Paris VI
“DNA obtained from using the ZymoPURE kit was ten times the amount of DNA obtained from using the QIAGEN kit.”
J. M., Vanderbilt University
“Thank you for letting me try this product out. I have become a new Zymo fan. Keep up the good work you guys are doing over there.”
L. T., USDA
“I loved the lack of an alcohol precipitation/centrifugation step!!! I also loved eluting directly into an EPPY tube at the end. This is VERY NICE.”
C. M., University of Georgia
“Thanks for letting me to try this product. I am very much happy with your awesome innovation. I am a big fan of your products because right now I am using 1) Zyme ChIP clean up kit. 2) DNA clean up KIt. 3) Gel Purification Kit. Now this kit.”
J. M., Umeå University
“Great product! Very convenient and fool proof. I would feel comfortable to let interns or people with little experience using this kit and still getting great results.”
X. L., University of Alabama at Birmingham
“I was very satisfied with this product. It produced high DNA concentration eluting in 400 µl using the above mentioned cell line, which would generally only be obtainable with other kits eluting in much smaller volumes. This product is also miles ahead of other general Maxiprep kits in terms of time required for plasmid extraction.”
J. P., University of Pretoria
“We were impressed by the high yield of plasmid DNA from this kit! From a 150 mL culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”
C. S., Abbott Laboratories
“Perfect! Finally, we got 600ug plasmid from 150ml E.coli culture in half an hour.”
B. P., Los Angeles Biomedical Research Institute at Harbor-UCLA
Click here to submit your review of the ZymoPURE™ Plasmid Maxiprep Kit.