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Zyppy™-96 Plasmid Miniprep

The fastest and simplest high-throughput procedures for purifying high-quality plasmid DNA.
Innovative pellet-free procedures and in-situ lysis omits cell pelleting and resuspension steps.
Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.

Product Size Catalog # Price Qty
Zyppy™-96 Plasmid Miniprep 2x96 Preps. D4041
$370.00
Zyppy™-96 Plasmid Miniprep 4x96 Preps D4042
$662.00
Zyppy™-96 Plasmid Miniprep 8x96 Preps D4043
$1,190.00

About Zyppy™-96 Plasmid Miniprep

The Zyppy™-96 Plasmid Miniprep is the fastest and simplest high-throughput method available for efficient isolation of plasmid DNA from E. coli. The kit features a modified, Pellet-Free alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization/Clearing Buffer. The remaining DNA purification steps are straightforward and simple. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.


Format 96-well
Processing Time 45 min
DNA Purity Eluted plasmid DNA is well suited for ligation, sequencing, restriction endonuclease digestion, transfection, in vitro transcription, and other sensitive applications requiring pure DNA. Abs260/280 is ≥1.8
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 10 µg per preparation, depending on the plasmid copy number, culture growth conditions, and the strain of E. Coli processed.
Procedure Performed at room temperature (15-30°C) with a centrifuge with micro plate carriers.
Recovery Volume ≥ 30 µl per well

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“Performed side-by-side preparations of single culture split into two, using the Zyppy and a leading competitor's miniprep kit. The Zyppy kit saved me ~25 minutes and returned plasmid of better 260/280.”
Greg G. (North Carolina State University)
“Fast, efficient purification, good with elution of concentrated DNA thanks to a different spin column design, fewer buffers and fewer steps than in other minprep protocols, all buffers prepared and ready to use straight out of the package, good price. Please don't change anything, the kit works great.”
Sebastian K (Lund University)
“Simple procedure was easy to follow, high yield. Blue/yellow color visualization. In spite of all the advantages of above, it is inexpensive!!!”
Hyejin Y. (Washington University School of Medicine.)
“It's so FAST! And it works!! I can't think of a way to improve this product. My big problem with it is that it is SO fast that I don't have time to label my tubes!”
Anita F. (Fairfield University)
“The kit gives a very good yield of plasmid DNA of up to 12 kb in size. I like the fact that procedure can be modified for purification of plasmid DNA from up to 3 ml of bacterial culture.”
Vladimir B (Touro University Nevada)
“The quality of the DNA is very good. Sequencing reads are generally beautiful.”
Magdalena B. (University of Massachusetts, Amherst)
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
Richard F.
The results gave what they promised! Clean DNA.
Pamela R (UT Medicine San Antonio)
Click here to submit your review of the Zyppy™-96 Plasmid Miniprep .

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