Zyppy™ Plasmid Midiprep Kit

The fastest, simplest midiprep available for purifying transfection quality plasmid DNA.
Pellet-free procedure omits conventional cell pelleting and resuspension steps.
DNA quality appropriate for cloning, sequencing, and transfection.

Product Size Catalog # Price Qty
Zyppy™ Plasmid Midiprep Kit 25 Preps D4025
$181.00
Zyppy™ Plasmid Midiprep Kit 50 Preps D4026
$325.00

About Zyppy™ Plasmid Midiprep Kit

The Zyppy™ Plasmid Midiprep Kit is a large-scale (up to 100 µg DNA) version of the Zyppy™ Plasmid Miniprep Kit. It features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Midiprep Kit is the fastest and simplest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.


Format Spin Column
Processing Time 15 min
DNA Purity Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8.
Elution Volume ≥ 150 µl
Plasmid DNA Size Up to 25 kb.
Plasmid DNA Yield ≥ 50 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Procedure Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold.

 

DNA yield from Zyppy™ Plasmid Midiprep Kit and a kit from Supplier A. EcoRI digestion of plasmid DNA (pGEM®) isolated from a 6 ml E. Coli culture using the Zyppy™ Plasmid Midiprep Kit or a kit from Supplier A. Performed in duplicate. M, ZR 1 kb DNA Marker (Zymo Research).
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Featured Citations

Zyppy Plasmid Midiprep and Maxiprep kits were used to prepare amplified recombinant plasmids containing previously uncharacterized endosome and vacuole interface genes from E. coli. Efficient preparation of recombinant plasmids was essential in the identification of unique genes involved in lysosomal vacuole function and morphology in yeast.

“Fast, efficient purification, good with elution of concentrated DNA thanks to a different spin column design, fewer buffers and fewer steps than in other minprep protocols, all buffers prepared and ready to use straight out of the package, good price. Please don't change anything, the kit works great.”
Sebastian K (Lund University)
“Simple procedure was easy to follow, high yield. Blue/yellow color visualization. In spite of all the advantages of above, it is inexpensive!!!”
Hyejin Y. (Washington University School of Medicine.)
“It's so FAST! And it works!! I can't think of a way to improve this product. My big problem with it is that it is SO fast that I don't have time to label my tubes!”
Anita F. (Fairfield University)
“The kit gives a very good yield of plasmid DNA of up to 12 kb in size. I like the fact that procedure can be modified for purification of plasmid DNA from up to 3 ml of bacterial culture.”
Vladimir B (Touro University Nevada)
“The quality of the DNA is very good. Sequencing reads are generally beautiful.”
Magdalena B. (University of Massachusetts, Amherst)
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
Richard F.
The results gave what they promised! Clean DNA.
Pamela R (UT Medicine San Antonio)
Click here to submit your review of the Zyppy™ Plasmid Midiprep Kit .