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Highlights
- Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
- RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
- Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
Description
| 1Unit Definition | One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz. |
|---|---|
| 2Storage | After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C). |
| 3Reconstitution | Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators. |
| 4Inactivation | Heat inactivate at 75 °C for 10 min with 5 mM EDTA. |
- Remove contaminating DNA from RNA or protein samples
- Clean up of RNA samples prior to RT-PCR
- DNA-free RNA sample preparation prior to RNA-seq
- Remove DNA templates after in vitro transcription
- DNA labeling by nick-translation
- Reduce viscosity in cell culture or cell lysate
- DNase I footprinting
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $17.00 | |
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $32.90 | |