About Mix & Go Competent Cells - Strain JM109
The Mix & Go E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go competent cells are highly efficient (> 108 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Premade Mix & Go competent cells strain JM109 are supplied as a pack of 10 convenient single use aliquots or as a 96-well rack with removable 8-tube strips for your high-throughput transformation needs.
|Format||Either as a pack of ten single use aliquots (100 µl / tube) or as a 96-tube rack with removable 8-tube strips (50 µl / tube)|
|Genotype||F`[traD36 proA+B+ laclq ∆(lacZ)M15] ∆(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1|
|Additional Information||Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g., λgt11. Can be used for M13 cloning/sequencing and blue/white screening.|
The authors found that the narrow-spectrum mercury resistance (mer) operon of Tenacibaculum discolor encode a novel ArsR/SmtB family transcriptional regulator that controls the expression of a mercuric reductase. To confirm that the ArsR/SmtB transcriptional regulator was mercury-responsive, a reporter plasmid was constructed by replacing the reductase gene with a luciferase gene and transformed into Mix & Go Competent E. coli cells, strain JM109, from Zymo Research. The purified plasmid was used to carry out a functional assay that showed the regulator can be stimulated by low concentrations of mercury.