5-hmC Glucosyltransferase Overview
Several methods to investigate DNA hydroxymethylation require 5-hmC-modified bases to first be glycosylated in vitro. Specific glucosylation of 5-hmC can be easily accomplished by the highly active 5-hmC Glucosyltransferase enzyme from Zymo Research. J-Binding Protein-1 (JBP-1) specifically binds to glucosylated DNA with high affinity, allowing 5-hmC levels to be determined by JBP-1-based enrichment. Additionally, glucosylation of 5-hmC alters the digestion of DNA by several restriction enzymes, and therefore digestion patterns of 5-hmC-glucosylated DNA can be used to assess DNA hydroxymethylation levels.