Specifications
| Concentration | 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
|---|---|
| Applications | Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions. |
| Genotype | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
| Source | Escherichia coli strain ER2925* (Dam"“ and Dcm"“) [*Used with permission from New England Biolabs.] |
