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Global 5-mC Quantification Overview

For analysis of DNA methylation levels by high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), or liquid chromatography-mass spectrometry (LC-MS), it is essential to completely degrade DNA to its individual nucleoside or nucleotide components. Zymo Research offers highly active enzymes that quickly, efficiently, and completely digest DNA to either individual nucleotides (5’-triphosphate, base, and sugar) or nucleosides (base and sugar, without 5’-triphosphate).

Global 5-mC Quantification Technologies

DNA Degradase™

Completely degrade DNA into individual nucleotides.

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DNA Degradase Plus™

Completely degrade DNA into individual nucleosides.

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