About Methylated-DNA IP Kit
The Methylated-DNA IP Kit utilizes immunoprecipitation technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the capture and separation of methylated DNA from non-methylated DNA in only a few hours (see figure below). Typically, over a hundred-fold enrichment of methylated DNA vs. non-methylated DNA can be achieved with the use of this kit. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. The product is provided with control DNA and primers for use as a control.
Specifications
| Format | Magnetic Beads |
|---|---|
| Processing Time | 3 hrs |
| Applications | Utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA with a highly specific anti-5-methylcytosine monoclonal antibody for the "capture" and separation of methylated DNA from non-methylated DNA in only a few hours, from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. |
| Components | Comes as a two component kit: the kit box along with a separate icebox containing the cold-storage components. |
| Enrichment Factor | > 100 fold for Methylated versus Non-methylated DNA. |
| Optimal DNA Input | 50 - 500 ng |
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