OneStep qMethyl™ Array

Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms. Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR (MSP), HPLC, and Methylated DNA Immunoprecipitation (MeDIP) have proven too costly, time consuming, or inappropriate for the investigation of large numbers of regions. Alternatively, each OneStep qMethyl™ Array from Zymo Research provides a simple, straightforward, and bisulfite-free procedure for rapid, DNA methylation assessment in the promoter region of any one of the following prominent tumor suppressor genes: RASSF1, RARB, CDKN2A (p16), MGMT, or CCND2. The reagents in the plate (array) are already premixed and optimized for robust amplification and detection. Simply add DNA into the appropriate well and then quantitate via real-time PCR… OneStep!



View a poster about the OneStep qMethyl™ Array